Comparison of DNA extraction methods for non-marine molluscs: is modified CTAB DNA extraction method more efficient than DNA extraction kits?
نویسندگان
چکیده
منابع مشابه
An Efficient Method For DNA Extraction From Paraffin Wax Embedded Tissues For PCR Amplification Of Human And Viral DNA
Background and Objective: Formalin-fixed paraffin-embedded tissues are a valuable source of DNA for molecular studies. We designed and optimized an efficient procedure for DNA extraction from formalin-fixed paraffin embedded tissues. Materials and Methods: Seventy three blocks of cervical paraffin-embedded tissues were investigated. DNA was extracted using 45 minutes boiling in alkaline sol...
متن کاملEvaluation and comparison of FTA card and CTAB DNA extraction methods for non-agricultural taxa1
PREMISE OF THE STUDY An efficient, effective DNA extraction method is necessary for comprehensive analysis of plant genomes. This study analyzed the quality of DNA obtained using paper FTA cards prepared directly in the field when compared to the more traditional cetyltrimethylammonium bromide (CTAB)-based extraction methods from silica-dried samples. METHODS DNA was extracted using FTA cards...
متن کاملAssesment of different DNA extraction methods in medicinal plants
Medicinal plants have high importance in medicine and pharmacogonosy because of their secondary metabolites. Essential oils, antioxidants and flavonoids are the major compounds of many medicinal plants. These compounds especially antioxidants can increase oxidation of DNA in response to wounds. Nowadays, progresses in biotechnology such as molecular markers were used for phylogenetic relationsh...
متن کاملAssesment of different DNA extraction methods in medicinal plants
Medicinal plants have high importance in medicine and pharmacogonosy because of their secondary metabolites. Essential oils, antioxidants and flavonoids are the major compounds of many medicinal plants. These compounds especially antioxidants can increase oxidation of DNA in response to wounds. Nowadays, progresses in biotechnology such as molecular markers were used for phylogenetic relationsh...
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van der Zee and Crielaard recently reported the contamination of Qiagen DNA extraction columns with Legionella species (6). We have independently made this discovery and have attempted to further characterize the contaminating DNA. We used the Qiagen columns (QIAamp DNA minikit; Qiagen, Hilden, Germany) for the extraction of DNA from a variety of clinical samples to be tested for Legionella DNA...
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ژورنال
عنوان ژورنال: 3 Biotech
سال: 2020
ISSN: 2190-572X,2190-5738
DOI: 10.1007/s13205-020-2051-7